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membranes  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology membranes
    Membranes, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membranes/nardilysin+Antibody/pmc12811471-130-0-10
    Average 93 stars, based on 4 article reviews
    membranes - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle
    Article Snippet: .. Membranes were incubated with primary antibodies directed to NRDC (sc-137199; Santa Cruz Biotechnology, Dallas, TX, USA), AMP-activated protein kinase α (AMPKα) (#2532; Cell Signaling Technology, Danvers, MA, USA), Akt substrate of 160 kDa (AS160) (#2670; Cell Signaling Technology), phospho-AS160 (#8730; Cell Signaling Technology), protein kinase B (AKT) (#9272; Cell Signaling Technology), phospho-AKT (Ser473) (#9271; Cell Signaling Technology), or puromycin (MABE343; Merck Burlington, MA, USA). .. Membranes were incubated with species-appropriate horseradish peroxidase-conjugated secondary antibody before proteins were visualized by enhanced chemiluminescence (Amersham ECL Western Blotting Detection Reagent; GE Healthcare Life Sciences, Little Chalfont, UK).

    Article Title: Identification and validation of an interaction between the E3 Ubiquitin Ligase WWP1 and the transcriptional co-activator WBP2 in the human heart.
    Article Snippet: .. Membranes were then incubated at 4 ̊C overnight in primary antibodies (mouse anti-yeast GAL 4 DNA-binding domain, clone RK5C1, Santa Cruz Biotechnology #sc510, RRID:AB_627655, 1:200; mouse anti-human WWP1, Abnova #H00011059-M01, RRID:AB_509107, 1:500; rabbit anti-yeast -tubulin, clone EPR13799, Abcam #ab184970, RRID:AB_2928998,1:15,000) diluted in blocking buffer. .. Species specific HRP-conjugated secondary antibodies (sheep anti-mouse IgG #NA931, GE Healthcare or goat anti-rabbit IgG #7074S, Cell Signaling Technologies) and SuperSignal West Femto Maximum Signaling Substrate (ThermoFisher) were used for signal detection and membranes were imaged using a BioRad ChemiDoc Touch gel imaging system.

    Article Title: Synthesis and structure-activity relationship study of a library of 1-substituted [1,2,4]triazolo[4,3- a ]quinolines as valuable chemical probes for paraxonase-2 (PON2)
    Article Snippet: Membranes were blocked in 5% BSA (Sigma-Aldrich) prepared in PBS containing 0.1% Tween-20 (PBST). .. Membranes were incubated overnight at 4 °C with a mouse monoclonal anti-PON2 antibody (C-5, sc-374158; Santa Cruz Biotechnology; 1:1000). .. After washing with PBST, membranes were incubated for 1 h at room temperature with goat anti-mouse IgG (H + L) HRP-conjugated secondary antibody (Bio-Rad, catalogue #1706516) diluted 1:5000.

    Article Title: DKK1 exacerbates dental pulp inflammation: insights from RNA-sequencing analysis.
    Article Snippet: Dickkopf-related protein 1 (DKK1) has been implicated in several inflammatory diseases; however, its role in pulpitis remains poorly understood.. This study aimed to investigate the role and underlying molecular mechanisms of DKK1 on pulpitis using inflamed dental pulp cells (DPCs) as the experimental model. RNA-sequencing (RNA-seq) was conducted on varying concentrations of lipopolysaccharide (LPS)-treated DPCs.. Subsequent bioinformatic analyses revealed distinct transcriptional expression patterns between moderate inflammation induced by 1 μg/ml LPS and excessive inflammation induced by 10 μg/ml LPS.

    Article Title: Protective Effects of p-Coumaric Acid Against Paclitaxel-Induced Testicular Damage: Role of Oxidative Stress, Inflammation, Apoptosis, and Autophagy.
    Article Snippet: Paclitaxel (PTX), despite its widespread antineoplastic use, induces severe reproductive toxicity by triggering oxidative stress, inflammation, and apoptosis in testicular tissue.. In this study, the effects of p-Coumaric Acid (PCA) on oxidative stress, inflammation, apoptosis, and hormonal balance were evaluated at histopathological, biochemical, and molecular levels in a rat model of PTX-induced testicular injury.. PTX increases reactive oxygen species (ROS) production and suppresses antioxidant defenses, thereby elevating lipid peroxidation; enhances nuclear factor kappa-B (NF-κB) and Toll-like receptor 4 (TLR4)-mediated pro-inflammatory cytokines interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α); and reduces interleukin-10 (IL-10) levels.

    Article Title: Comparative evaluation of TRIDs : a strategy to improve treatments.
    Article Snippet: .. Membranes were incubated overnight at 4 °C with either anti-p53 antibody (DO1, 1:200; Santa Cruz Biotechnology) or anti-IMPORTIN9 antibody (1:1000; Abcam). .. Following three washes in TBS-T, membranes were probed with HRP-conjugated secondary antibodies specific for mouse (1:5000; Jackson ImmunoResearch) or rabbit (1:50,000) primary antibodies.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide.
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 ◦C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 °C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Blocking Assay:

    Article Title: Identification and validation of an interaction between the E3 Ubiquitin Ligase WWP1 and the transcriptional co-activator WBP2 in the human heart.
    Article Snippet: .. Membranes were then incubated at 4 ̊C overnight in primary antibodies (mouse anti-yeast GAL 4 DNA-binding domain, clone RK5C1, Santa Cruz Biotechnology #sc510, RRID:AB_627655, 1:200; mouse anti-human WWP1, Abnova #H00011059-M01, RRID:AB_509107, 1:500; rabbit anti-yeast -tubulin, clone EPR13799, Abcam #ab184970, RRID:AB_2928998,1:15,000) diluted in blocking buffer. .. Species specific HRP-conjugated secondary antibodies (sheep anti-mouse IgG #NA931, GE Healthcare or goat anti-rabbit IgG #7074S, Cell Signaling Technologies) and SuperSignal West Femto Maximum Signaling Substrate (ThermoFisher) were used for signal detection and membranes were imaged using a BioRad ChemiDoc Touch gel imaging system.

    Article Title: Protective Effects of p-Coumaric Acid Against Paclitaxel-Induced Testicular Damage: Role of Oxidative Stress, Inflammation, Apoptosis, and Autophagy.
    Article Snippet: Paclitaxel (PTX), despite its widespread antineoplastic use, induces severe reproductive toxicity by triggering oxidative stress, inflammation, and apoptosis in testicular tissue.. In this study, the effects of p-Coumaric Acid (PCA) on oxidative stress, inflammation, apoptosis, and hormonal balance were evaluated at histopathological, biochemical, and molecular levels in a rat model of PTX-induced testicular injury.. PTX increases reactive oxygen species (ROS) production and suppresses antioxidant defenses, thereby elevating lipid peroxidation; enhances nuclear factor kappa-B (NF-κB) and Toll-like receptor 4 (TLR4)-mediated pro-inflammatory cytokines interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α); and reduces interleukin-10 (IL-10) levels.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide.
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 ◦C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 °C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Software:

    Article Title: DKK1 exacerbates dental pulp inflammation: insights from RNA-sequencing analysis.
    Article Snippet: Dickkopf-related protein 1 (DKK1) has been implicated in several inflammatory diseases; however, its role in pulpitis remains poorly understood.. This study aimed to investigate the role and underlying molecular mechanisms of DKK1 on pulpitis using inflamed dental pulp cells (DPCs) as the experimental model. RNA-sequencing (RNA-seq) was conducted on varying concentrations of lipopolysaccharide (LPS)-treated DPCs.. Subsequent bioinformatic analyses revealed distinct transcriptional expression patterns between moderate inflammation induced by 1 μg/ml LPS and excessive inflammation induced by 10 μg/ml LPS.

    Membrane:

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide.
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 ◦C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 °C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Saline:

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide.
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 ◦C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 °C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Control:

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide.
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 ◦C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.

    Article Title: American Ginseng ( Panax quinquefolius ) Extracts (G1899) Ameliorate Immunosenescence via Regulation of T Cell Populations and Aging-Related Proteins in a Mouse Model Induced by D-Galactose and Tert-Butyl Hydroperoxide
    Article Snippet: Equal amounts of protein (30 μg/lane) were separated by SDS-PAGE on 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. To block the membrane, it was incubated for 2 h with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST), and membranes were probed with primary antibodies [anti-FOXO1 (1:1000, #2880), anti-Sirt1 (1:1000, #9475), anti-p53 (1:1000, #2527), and anti-CD38 (1:1000, #14637) (all from Cell Signaling Technology, Danvers, MA, USA)] for 24 h at 4 °C. β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA) served as an internal control. .. Protein signals were developed on the membranes using ECL substrates (Thermo Fisher Scientific) and analyzed using a Fusion FX6.0 (Vilber, Collégien, France) system.



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    Servicebio Inc m1 membrane
    Schematic diagram of the preparation and anticancer mechanism <t>of</t> <t>M1@CAT-Ce6-Rh2</t> Lips. (A) Schematic illustration of the preparation procedure for M1 macrophage membrane-camouflaged self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). Briefly, Nanoparticles were prepared using the anticancer drug Rh2 as the membrane material to encapsulate the photosensitizer Ce6 and CAT, which were then coated with lipopolysaccharide (LPS)-stimulated M1 macrophage membrane to enhance uptake by tumor cells. (B) Schematic depiction of the in vivo fate and mechanism of antineoplastic activity of M1@CAT-Ce6-Rh2 Lips upon intravenous administration. These biomimetic nanoparticles possess prolonged systemic circulation duration and preferential enrichment within tumor regions through the enhanced permeability and retention (EPR) effect. Following internalization into cancer cells, each functional component exerts its unique biological activity, synergistically triggering ferroptosis in cancer cells and ultimately exerting potent antitumor efficacy.
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    Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Journal: Journal of Sport and Health Science

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

    doi: 10.1016/j.jshs.2025.101095

    Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Article Snippet: Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane

    Schematic diagram of the preparation and anticancer mechanism of M1@CAT-Ce6-Rh2 Lips. (A) Schematic illustration of the preparation procedure for M1 macrophage membrane-camouflaged self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). Briefly, Nanoparticles were prepared using the anticancer drug Rh2 as the membrane material to encapsulate the photosensitizer Ce6 and CAT, which were then coated with lipopolysaccharide (LPS)-stimulated M1 macrophage membrane to enhance uptake by tumor cells. (B) Schematic depiction of the in vivo fate and mechanism of antineoplastic activity of M1@CAT-Ce6-Rh2 Lips upon intravenous administration. These biomimetic nanoparticles possess prolonged systemic circulation duration and preferential enrichment within tumor regions through the enhanced permeability and retention (EPR) effect. Following internalization into cancer cells, each functional component exerts its unique biological activity, synergistically triggering ferroptosis in cancer cells and ultimately exerting potent antitumor efficacy.

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: Schematic diagram of the preparation and anticancer mechanism of M1@CAT-Ce6-Rh2 Lips. (A) Schematic illustration of the preparation procedure for M1 macrophage membrane-camouflaged self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). Briefly, Nanoparticles were prepared using the anticancer drug Rh2 as the membrane material to encapsulate the photosensitizer Ce6 and CAT, which were then coated with lipopolysaccharide (LPS)-stimulated M1 macrophage membrane to enhance uptake by tumor cells. (B) Schematic depiction of the in vivo fate and mechanism of antineoplastic activity of M1@CAT-Ce6-Rh2 Lips upon intravenous administration. These biomimetic nanoparticles possess prolonged systemic circulation duration and preferential enrichment within tumor regions through the enhanced permeability and retention (EPR) effect. Following internalization into cancer cells, each functional component exerts its unique biological activity, synergistically triggering ferroptosis in cancer cells and ultimately exerting potent antitumor efficacy.

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: Membrane, In Vivo, Activity Assay, Permeability, Functional Assay

    Physicochemical characterization of biomimetic self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). (A) Schematic illustration of the preparation of biomimetic nanoparticles; (B) UV-Vis absorption spectrum of M1@CAT-Ce6-Rh2 Lips; (C) FT-IR spectrum of Ce6-Rh2 Lips; (D) Representative particle size and ζ-potential profiles of M1@CAT-Ce6-Rh2 Lips; (E) Quantitative analysis of particle size for different formulations (n = 3); (F) Quantitative analysis of PDI values (n = 3); (G) Quantitative analysis of ζ-potential (n = 3); (H) Encapsulation efficiency of different formulations (n = 3); (I) TEM image of M1@CAT-Ce6-Rh2 Lips, scale bar = 50 nm; (J) SDS-PAGE analysis of protein profiles for CAT-Ce6-Rh2 Lips, M1@CAT-Ce6-Rh2 Lips, and M1 macrophage membrane; (K) Western blot analysis of integrin α4 and integrin β1 expression in M1 macrophages, M1 macrophage membrane, and M1@CAT-Ce6-Rh2 Lips; (L – O) Stability of different formulations after storage at 4 °C for 28 days (L, M) or incubation in serum for 48 h (N, O). Changes in encapsulation efficiency (L, N) and particle size (M, O) were quantitatively analyzed. Data are shown as mean ± SD (n = 3); (P) In vitro release profiles of Ce6 from various formulations; (Q) Time-dependent relative catalytic activities of free CAT, CAT-Ce6-Rh2 Lips, and M1@CAT-Ce6-Rh2 Lips upon proteinase K treatment; (R) Hemolytic activity of different formulations, where “−” and “+” indicate negative and positive controls, respectively.

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: Physicochemical characterization of biomimetic self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). (A) Schematic illustration of the preparation of biomimetic nanoparticles; (B) UV-Vis absorption spectrum of M1@CAT-Ce6-Rh2 Lips; (C) FT-IR spectrum of Ce6-Rh2 Lips; (D) Representative particle size and ζ-potential profiles of M1@CAT-Ce6-Rh2 Lips; (E) Quantitative analysis of particle size for different formulations (n = 3); (F) Quantitative analysis of PDI values (n = 3); (G) Quantitative analysis of ζ-potential (n = 3); (H) Encapsulation efficiency of different formulations (n = 3); (I) TEM image of M1@CAT-Ce6-Rh2 Lips, scale bar = 50 nm; (J) SDS-PAGE analysis of protein profiles for CAT-Ce6-Rh2 Lips, M1@CAT-Ce6-Rh2 Lips, and M1 macrophage membrane; (K) Western blot analysis of integrin α4 and integrin β1 expression in M1 macrophages, M1 macrophage membrane, and M1@CAT-Ce6-Rh2 Lips; (L – O) Stability of different formulations after storage at 4 °C for 28 days (L, M) or incubation in serum for 48 h (N, O). Changes in encapsulation efficiency (L, N) and particle size (M, O) were quantitatively analyzed. Data are shown as mean ± SD (n = 3); (P) In vitro release profiles of Ce6 from various formulations; (Q) Time-dependent relative catalytic activities of free CAT, CAT-Ce6-Rh2 Lips, and M1@CAT-Ce6-Rh2 Lips upon proteinase K treatment; (R) Hemolytic activity of different formulations, where “−” and “+” indicate negative and positive controls, respectively.

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: Encapsulation, SDS Page, Membrane, Western Blot, Expressing, Incubation, In Vitro, Activity Assay

    In vitro targeting evaluation of biomimetic self-oxygenating M1@CAT-Ce6-Rh2 Lips. (A) Flow cytometric analysis of the uptake of different formulations by LLC cells; (B) Quantitative analysis of relative cellular fluorescence intensity by flow cytometry; (C) Fluorescence microscopy images showing the uptake of different formulations by LLC cells; (D) Quantitative analysis of relative cellular fluorescence intensity from fluorescence microscopy images. Scale bar = 50 μm. Data are presented as mean ± standard deviation (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: In vitro targeting evaluation of biomimetic self-oxygenating M1@CAT-Ce6-Rh2 Lips. (A) Flow cytometric analysis of the uptake of different formulations by LLC cells; (B) Quantitative analysis of relative cellular fluorescence intensity by flow cytometry; (C) Fluorescence microscopy images showing the uptake of different formulations by LLC cells; (D) Quantitative analysis of relative cellular fluorescence intensity from fluorescence microscopy images. Scale bar = 50 μm. Data are presented as mean ± standard deviation (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: In Vitro, Fluorescence, Flow Cytometry, Microscopy, Standard Deviation

    In vitro anti-tumor pharmacodynamic evaluation of biomimetic self-oxygenating M1@CAT-Ce6-Rh2 Lips. (A – B) Representative colony formation images and quantitative analysis of the colony formation rate; (C – D) Representative EDU proliferation images (scale bar: 50 μm) and quantitative analysis of the proliferation rate; (E – F) Representative live/dead staining images (scale bar: 50 μm) and corresponding quantitative analysis; (G – H) Flow cytometric analysis of apoptosis and quantitative determination of the apoptosis rate. Data are presented as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: In vitro anti-tumor pharmacodynamic evaluation of biomimetic self-oxygenating M1@CAT-Ce6-Rh2 Lips. (A – B) Representative colony formation images and quantitative analysis of the colony formation rate; (C – D) Representative EDU proliferation images (scale bar: 50 μm) and quantitative analysis of the proliferation rate; (E – F) Representative live/dead staining images (scale bar: 50 μm) and corresponding quantitative analysis; (G – H) Flow cytometric analysis of apoptosis and quantitative determination of the apoptosis rate. Data are presented as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: In Vitro, Staining, Standard Deviation

    Investigation of the in vitro antitumor mechanism of M1@CAT-Ce6-Rh2 Lips. (A – B) Representative images of total ROS staining and quantitative analysis of fluorescence intensity; (C – D) Intracellular ROS levels detected by flow cytometry and flow quantitative analysis; (E – F) Representative images of 1 O 2 detected by SOSG staining and quantitative analysis of fluorescence intensity; (G – H) Representative images of O 2 − · detected by DHE staining and quantitative analysis of fluorescence intensity; (I – J) Immunofluorescence staining images of SLC7A11 and quantitative analysis of fluorescence intensity; (K – L) Western blot and quantitative analysis of SLC7A11. Data are presented as mean ± standard deviation (n = 3).*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significant difference.

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: Investigation of the in vitro antitumor mechanism of M1@CAT-Ce6-Rh2 Lips. (A – B) Representative images of total ROS staining and quantitative analysis of fluorescence intensity; (C – D) Intracellular ROS levels detected by flow cytometry and flow quantitative analysis; (E – F) Representative images of 1 O 2 detected by SOSG staining and quantitative analysis of fluorescence intensity; (G – H) Representative images of O 2 − · detected by DHE staining and quantitative analysis of fluorescence intensity; (I – J) Immunofluorescence staining images of SLC7A11 and quantitative analysis of fluorescence intensity; (K – L) Western blot and quantitative analysis of SLC7A11. Data are presented as mean ± standard deviation (n = 3).*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significant difference.

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: In Vitro, Staining, Fluorescence, Flow Cytometry, Immunofluorescence, Western Blot, Standard Deviation

    Investigation of the relationship between ferroptosis and the antitumor mechanism of M1@CAT-Ce6-Rh2 Lips. (A) Transmission electron microscopy images of mitochondria, → Mitochondrial double membrane; ▲ Mitochondrial cristae; (B) Quantitative analysis of GSH content; (C – D) Western blot and quantitative analysis of GPX4; (E – F) Representative immunofluorescence images and fluorescence intensity analysis of GPX4; (G) Quantitative analysis of MDA content; (H – I) Immunofluorescence staining images and quantitative analysis of 4-HNE; (J – K) C11-BODIPY staining images and quantitative analysis of lipid peroxidation; (L – M) FerroOrange Fe 2+ fluorescence images and quantitative analysis; (N – Q) Mito-Tracker Red (mitochondrial membrane potential, red) and JC-1 staining and corresponding quantitative analysis; (R – S) Staining images and quantitative analysis of mitochondrial permeability transition pore opening. Data are presented as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: Investigation of the relationship between ferroptosis and the antitumor mechanism of M1@CAT-Ce6-Rh2 Lips. (A) Transmission electron microscopy images of mitochondria, → Mitochondrial double membrane; ▲ Mitochondrial cristae; (B) Quantitative analysis of GSH content; (C – D) Western blot and quantitative analysis of GPX4; (E – F) Representative immunofluorescence images and fluorescence intensity analysis of GPX4; (G) Quantitative analysis of MDA content; (H – I) Immunofluorescence staining images and quantitative analysis of 4-HNE; (J – K) C11-BODIPY staining images and quantitative analysis of lipid peroxidation; (L – M) FerroOrange Fe 2+ fluorescence images and quantitative analysis; (N – Q) Mito-Tracker Red (mitochondrial membrane potential, red) and JC-1 staining and corresponding quantitative analysis; (R – S) Staining images and quantitative analysis of mitochondrial permeability transition pore opening. Data are presented as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: Transmission Assay, Electron Microscopy, Membrane, Western Blot, Immunofluorescence, Fluorescence, Staining, Permeability, Standard Deviation

    Evaluation of in vivo long-circulating effect and tumor targeting ability of various formulations. (A – B) Observation of the long-circulating effect in mice intravenously injected with different formulations via the tail vein; quantitative analysis of fluorescence signal intensity in blood; (C) Real-time in vivo fluorescence images of tumor-bearing mice detected at demonstrate that nano-formulation, especially M1 macrophage membrane-based modification, can effectively prolong the blood circulation time of Ce6, thereby providing a sufficient time window predetermined time points after intravenous administration of various formulations; (D) Ex vivo fluorescence imaging of major organs and tumors 96 h after intravenous administration (n = 3).

    Journal: Materials Today Bio

    Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

    doi: 10.1016/j.mtbio.2026.103327

    Figure Lengend Snippet: Evaluation of in vivo long-circulating effect and tumor targeting ability of various formulations. (A – B) Observation of the long-circulating effect in mice intravenously injected with different formulations via the tail vein; quantitative analysis of fluorescence signal intensity in blood; (C) Real-time in vivo fluorescence images of tumor-bearing mice detected at demonstrate that nano-formulation, especially M1 macrophage membrane-based modification, can effectively prolong the blood circulation time of Ce6, thereby providing a sufficient time window predetermined time points after intravenous administration of various formulations; (D) Ex vivo fluorescence imaging of major organs and tumors 96 h after intravenous administration (n = 3).

    Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

    Techniques: In Vivo, Injection, Fluorescence, Formulation, Membrane, Modification, Ex Vivo, Imaging